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This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer.
For more information, go to www. Assay Procedure Refer to the product for complete assay procedure. Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
Prepare all reagents, standard dilutions, and samples as directed in the product insert. Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
Aspirate each well and wash, repeating once more for a total of 2 washes. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker. Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
Aspirate and wash 4 times. Incubate at room temperature for 30 minutes on the benchtop. Read at nm within 30 minutes. Set wavelength correction to nm or nm. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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